بررسی خون سازگاری سطح مزانشیمی پرده‌ی آمنیون انسانی در مقایسه با رگ مصنوعی پوشش داده شده با هپارین

Background and Objective: Amniotic membrane (AM) as a natural tissue has lots of unique features which make it a suitable candidate for vascular tissue engineering. The aim of this study was to evaluate blood compatibility of mesenchymal surface of the AM. Materials and Methods: In this study, the effect of mesenchymal surface of the AM on internal and external coagulation pathways, hemolysis and platelet activity was measured and the results were compared with heparin-coated ePTFE as a synthetic vessel substitute. In addition, platelet adhesion and their morphologic changes after being in contact with samples were analyzed by electron microscopy. Results: Prothrombin time (PT), activated partial thromboplastin time (aPTT), clotting time and hemolysis tests showed that the AM is hemocompatible. Additionally, mesenchymal surface of the AM induced platelet aggregation less than ePTFE while both similarly provoked secretion of P-selectin. Number of adhered platelets to mesenchymal surface of the AM was less than ePTFE. The platelets on the mesenchymal surface had round morphology whereas ePTFE exhibited dendritic shape platelets. Conclusion: Results of this study showed that the mesenchymal surface of the AM is hemocompatible which could be a proper candidate for vascular tissue engineering. 1- Hoshi RA, Van Lith R, Jen MC, Allen JB, Lapidos KA, Ameer G. The blood and vascular cell compatibility of heparin-modified ePTFE vascular grafts. Biomaterials. 2013 34: 30-41. 2- Zhang WJ, Liu W, Cui L, Cao Y. Tissue engineering of blood vessel. J Cell Mol Med. 2007 11: 945-57. 3- Palumbo VD, Bruno A, Tomasello G, Damiano G, Lo Monte AI. Bioengineered vascular scaffolds: the state of the art. Int J Artif Organs. 2014 37: 503-12. 4- Suma H. Arterial grafts in coronary bypass surgery. Ann Thorac Cardiovasc Surg. 1999 5: 141-5. 5- Carnagey J, Hern-Anderson D, Ranieri J, Schmidt CE. Rapid endothelialization of photo fix natural biomaterial vascular grafts. J Biomed Mater Res B Appl Biomater. 2003 65: 171-9. 6- Seifu DG, Purnama A, Mequanint K, Mantovani D. Small-diameter vascular tissue engineering. Nat Rev Cardiol. 2013 10: 410-21. 7- Niknejad H, Deihim T, Ahmadiani A, Jorjani M, Peirovi H. Permanent expression of midbrain dopaminergic neurons traits in differentiated amniotic epithelial cells. Neurosci Lett. 2012 506: 22-7. 8- Niknejad H, Peirovi H, Jorjani M, Ahmadiani A, Ghanavi J, Seifalian AM. Properties of the amniotic membrane for potential use in tissue engineering. Eur Cell Mater. 2008 15: 88-99. 9- Tehrani FA, Ahmadiani A, Niknejad H. The effects of preservation procedures on antibacterial property of amniotic membrane. Cryobiology. 2013 67: 293-8. 10- Niknejad H, Khayat-Khoei M, Peirovi H. Inhibition of MMPs might increase anticancer properties of amniotic epithelial cells. Med Hypotheses. 2012 78: 690-1. 11- Niknejad H, Yazdanpanah G. Anticancer effects of human amniotic membrane and its epithelial cells. Med Hypotheses. 2014 82: 488-9. 12- Lee SB, Li DQ, Tan DT, Meller DC, Tseng SC. Suppression of TGF-beta signaling in both normal conjunctival fibroblasts and pterygial body fibroblasts by amniotic membrane. Curr Eye Res. 2000 20: 325-34. 13- Solomon A, Rosenblatt M, Monroy D, Ji Z, Pflugfelder SC, Tseng SC. Suppression of interleukin 1alpha and interleukin 1beta in human limbal epithelial cells cultured on the amniotic membrane stromal matrix. Br J Ophthalmol. 2001 85: 444-9. 14- Fernandes M, Sridhar MS, Sangwan VS, Rao GN. Amniotic membrane transplantation for ocular surface reconstruction. Cornea. 2005 24: 643-53. 15- Niknejad H, Deihim T, Solati-Hashjin M, Peirovi H. The effects of preservation procedures on amniotic membrane's ability to serve as a substrate for cultivation of endothelial cells. Cryobiology. 2011 63: 145-51. 16- Peirovi H, Rezvani N, Hajinasrollah M, Mohammadi SS, Niknejad H. Implantation of amniotic membrane as a vascular substitute in the external jugular vein of juvenile sheep. J Vasc Surg. 2012 56: 1098-104. 17- Niknejad H, Deihim T, Peirovi H, Abolghasemi H. Serum-free cryopreservation of human amniotic epithelial cells before and after isolation from their natural scaffold. Cryobiology. 2013 67: 56-63. 18- Ko TM, Lin JC, Cooper SL. Surface characterization and platelet adhesion studies of plasma-sulphonated polyethylene. Biomaterials. 1993 14: 657-64. 19- Goodman SL. Sheep, pig, and human platelet-material interactions with model cardiovascular biomaterials. J Biomed Mater Res. 1999 45: 240-50. 20- Motlagh D, Allen J, Hoshi R, Yang J, Lui K, Ameer G. Hemocompatibility evaluation of poly(diol citrate) in vitro for vascular tissue engineering. J Biomed Mater Res A. 2007 82: 907-16. 21- Niknejad H, Khayat-Khoei M, Peirovi H, Abolghasemi H. Human amniotic epithelial cells induce apoptosis of cancer cells: a new anti-tumor therapeutic strategy. Cytotherapy. 2014 16: 33-40. 22- Niknejad H, Yazdanpanah G, Mirmasoumi M, Abolghasemi H, Peirovi H, Ahmadiani A. Inhibition of HSP90 could be possible mechanism for anti-cancer property of amniotic membrane. Med Hypotheses. 2013 81: 862-5. 23- Niknejed H, Yazdanpanah G, Khayat-khoei M. In vitro evaluation of the effects of amniotic membrane on viability and proliferation of cancer cells. J Zanjan Unive Med Sci. 2013 21: 13-21. 24- Lin WC, Liu TY, Yang MC. Hemocompatibility of polyacrylonitrile dialysis membrane immobilized with chitosan and heparin conjugate. Biomaterials. 2004 25: 1947-57. 25- Wennberg A, Hensten-Pettersen A. Sensitivity of erythrocytes from various species to in vitro hemolyzation. J Biomed Mater Res. 1981 15: 433-5. 26- Hao Y, Ma DH, Hwang DG, Kim WS, Zhang F. Identification of antiangiogenic and antiinflammatory proteins in human amniotic membrane. Cornea. 2000 19: 348-52. 27- Murdoch AD, Dodge GR, Cohen I, Tuan RS, Iozzo RV. Primary structure of the human heparan sulfate proteoglycan from basement membrane (HSPG2/perlecan). A chimeric molecule with multiple domains homologous to the low density lipoprotein receptor, laminin, neural cell adhesion molecules, and epidermal growth factor. J Biol Chem. 1992 267: 8544-57. 28- Kuriu Y, Yamagishi H, Otsuji E, et al. Regeneration of peritoneum using amniotic membrane to prevent postoperative adhesions. Hepatogastroenterology. 2009 56: 1064-8. 29- Zaga-Clavellina V, Flores-Espinosa P, Pineda-Torres M, et al. Tissue-specific IL-10 secretion profile from term human fetal membranes stimulated with pathogenic microorganisms associated with preterm labor in a two-compartment tissue culture system. J Mater Fet Neo Med. 2014 27: 1320-7. 30- Choi G, Schultz MJ, Levi M, van der Poll T. The relationship between inflammation and the coagulation system. Swiss Med Wkly. 2006 136: 139-44. 31- Shao C, Sima J, Zhang SX, et al. Suppression of corneal neovascularization by PEDF release from human amniotic membranes. Invest Ophthalmol Vis Sci. 2004 45: 1758-62. 32- Lee Y-M, Lee J-J, Shen M-Y, Hsiao G, Sheu J-R. Inhibitory mechanisms of activated matrix metalloproteinase-9 on platelet activation. Eur J Pharmacol. 2006 537: 52-8. 33- Ilancheran S, Michalska A, Peh G, Wallace EM, Pera M, Manuelpillai U. Stem cells derived from human fetal membranes display multilineage differentiation potential. Biol Reprod. 2007 77: 577-88. 34- Oswald J, Boxberger S, Jorgensen B, et al. Mesenchymal stem cells can be differentiated into endothelial cells in vitro. Stem Cells. 2004 22: 377-84. 35- Niknejad H, Paeini-Vayghan G, Tehrani FA, Khayat-Khoei M, Peirovi H. Side dependent effects of the human amnion on angiogenesis. Placenta. 2013 34: 340-5. 36- Mitchell SL, Niklason LE. Requirements for growing tissue-engineered vascular grafts. Cardiovasc Pathol. 2003 12: 59-64.